• Salesperson：Panpan Wang（EFL）

  Phone：15335258233

  Email：engforlife-mkt@efl-tech.com

  Foreign distributor：ANR Technologies Pte Ltd

Introduction

Fluorescence-labeled hydrogel is a kind of hydrogel that can emit fluorescence at a specific wavelength under excitation light. This fluorescence characteristic makes it have broad application prospects in 3D printing, biosensing, disease diagnosis, fluorescence tracing and other research fields.

In 2019, the EFL team launched its fluorescent gelatin methacryloyl (EFL-GM-RF/GF/BF series), which makes the hydrogel take on different fluorescent colors by "grafting" fluorescent molecules on GelMA. Fluorescent GelMA has been well received by users and plays an important role in the study of gel structure and tracer degradation.

The market demand for fluorescent labeling of photo-crosslinkable hydrogels other than GelMA is increasing, with the continuous expansion of EFL’s photo-crosslinkable hydrogel product portfolio. In order to meet customer demand for fluorescent labeling of photo-crosslinkable hydrogel products such as HAMA, SilMA, AlgMA, DexMA, the EFL team has introduced Alkene coupling hydrogel fluorescent dyes (EFL-DYE-UF-ENE series) that can be grafted onto hydrogel polymer networks. The dyes are macromer monomers functionalized with vinyl groups and specific fluorescent molecules. After mixing with hydrogel precursors, they are coupled with gel polymer networks through free radical polymerization during photocuring to achieve stable fluorescence labeling. EFL-DYE-UF-ENE series of alkene coupling hydrogel fluorescent dyes are suitable for fluorescent labeling of photo-crosslinkable hydrogels. They have good biocompatibility and overcome the shortcomings of physical mixing or electrostatic adsorption methods in which fluorescent molecules are easy to diffuse out of the system. At the same time, they also avoid the shortcomings of uneven imaging of fluorescent particles.

Item

Fluorescence information

 Principle

Fig 1. The fluorescence principle characteristics and diffusion test of EFL-DYE-UF-ENE

Application

(1) Fluorescent labeling of 3D printing ink

Fig 2. Laser confocal images of 3D printed GelMA, HAMA, SilMA and other hydrogels after fluorescent labeling with EFL-DYE-UF-ENE series

(2)  Live imaging tracer

Fig 3. In vivo fluorescence imaging of the subcutaneous implant degradation of fluorescently labeled GelMA hydrogel (location: below the skin on the back of the mice; gel size: wafer of diameter 8mm and thickness 3mm; hydrogel: 5%GM60 using EFL-DYE-UF-ENE-R fluorescent dye)